Physiological adaptation of Desulfitobacterium hafniense strain tce1 to tetrachloroethene respiration

Laure Prat (1) , Julien Maillard (1) , R. Grimaud (2) , C. Holliger (1)

1 EPFL - Ecole Polytechnique Fédérale de Lausanne
2 IPREM - Institut des sciences analytiques et de physico-chimie pour l'environnement et les materiaux

Desulfitobacterium spp. are ubiquitous organisms with a broad metabolic versatility, and some isolates have the ability to use tetrachloroethene (PCE) as terminal electron acceptor. In order to identify proteins involved in this organohalide respiration process, a comparative proteomic analysis was performed. Soluble and membrane-associated proteins obtained from cells of Desulfitobacterium hafniense strain TCE1 that were growing on different combinations of the electron donors lactate and hydrogen and the electron acceptors PCE and fumarate were analyzed. Among proteins increasingly expressed in the presence of PCE compared to fumarate as electron acceptor, a total of 57 proteins were identified by mass spectrometry analysis, revealing proteins involved in stress response and associated regulation pathways, such as PspA, GroEL, and CodY, and also proteins potentially participating in carbon and energy metabolism, such as proteins of the Wood-Ljungdahl pathway and electron transfer flavoproteins. These proteomic results suggest that D. hafniense strain TCE1 adapts its physiology to face the relative unfavorable growth conditions during an apparent opportunistic organohalide respiration.

Keywords: Electron donors; Electron transfer; Growth conditions; Mass spectrometry analysis; Membrane-associated proteins; Metabolic versatility; Physiological adaptations; Proteomic analysis; two dimensional gel electrophoresis; Adaptation; Bacterial Proteins; Energy Metabolism; Lactic Acid; Metabolic Networks and Pathways; Oxidation-Reduction; Desulfitobacterium hafniense; physiological response; membrane; identification method; energy efficiency; comparative study; cell organelle; adaptive radiation; fumaric acid derivative; Terminal electron acceptors; Stress response

Domains: Bacteriology

Laure Prat, Julien Maillard, R. Grimaud, C. Holliger. Physiological adaptation of Desulfitobacterium hafniense strain tce1 to tetrachloroethene respiration. Applied and Environmental Microbiology, 2011, 77 (11), pp.3853-3859. ⟨10.1128/AEM.02471-10⟩. ⟨hal-01614788⟩