Physiological adaptation of Desulfitobacterium hafniense strain tce1 to tetrachloroethene respiration
Résumé
Desulfitobacterium spp. are ubiquitous organisms with a broad metabolic versatility, and some isolates have the ability to use tetrachloroethene (PCE) as terminal electron acceptor. In order to identify proteins involved in this organohalide respiration process, a comparative proteomic analysis was performed. Soluble and membrane-associated proteins obtained from cells of Desulfitobacterium hafniense strain TCE1 that were growing on different combinations of the electron donors lactate and hydrogen and the electron acceptors PCE and fumarate were analyzed. Among proteins increasingly expressed in the presence of PCE compared to fumarate as electron acceptor, a total of 57 proteins were identified by mass spectrometry analysis, revealing proteins involved in stress response and associated regulation pathways, such as PspA, GroEL, and CodY, and also proteins potentially participating in carbon and energy metabolism, such as proteins of the Wood-Ljungdahl pathway and electron transfer flavoproteins. These proteomic results suggest that D. hafniense strain TCE1 adapts its physiology to face the relative unfavorable growth conditions during an apparent opportunistic organohalide respiration.
Mots clés
Electron donors
Electron transfer
Growth conditions
Mass spectrometry analysis
Membrane-associated proteins
Metabolic versatility
Physiological adaptations
Proteomic analysis
two dimensional gel electrophoresis
Adaptation
Bacterial Proteins
Energy Metabolism
Lactic Acid
Metabolic Networks and Pathways
Oxidation-Reduction
Desulfitobacterium hafniense
physiological response
membrane
identification method
energy efficiency
comparative study
cell organelle
adaptive radiation
fumaric acid derivative
Terminal electron acceptors
Stress response
Domaines
Bactériologie
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