Graftable SCoMPIs enable the labeling and X-ray fluorescence imaging of proteins

Sarah Hostachy (1, 2, 3, 4, 5) , Marie Masuda (6) , Takayuki Miki (6) , Itaru Hamachi (6) , Sandrine Sagan (7, 2) , Olivier Lequin (7, 2) , Kadda Medjoubi (8) , Andrea Somogyi (8) , Nicolas Delsuc (1, 2, 9, 3, 5) , Clotilde Policar (1, 2, 9, 3, 5)

1 LBM-E1 - Peptides, glycoconjugués et métaux en biologie
2 LBM UMR 7203 - Laboratoire des biomolécules
3 INC-CNRS - Institut de Chimie - CNRS Chimie
4 ENS-PSL - École normale supérieure - Paris
5 PSL - Université Paris Sciences et Lettres
6 Kyoto University
7 LBM-E2 - Analyse, Interactions Moléculaires et Cellulaires
8 SSOLEIL - Synchrotron SOLEIL
9 Département de Chimie - ENS Paris

Bio-imaging techniques alternative to fluorescence microscopy are gaining increasing interest as complementary tools to visualize and analyze biological systems. Among them, X-ray fluorescence microspectroscopy provides information on the local content and distribution of heavy elements (Z ≥ 14) in cells or biological samples. In this context, similar tools to those developed for fluorescence microscopy are desired, including chemical probes or tags. In this work, we study rhenium complexes as a convenient and sensitive probe for X-ray fluorescence microspectroscopy. We demonstrate their ability to label and sense exogenously incubated or endogenous proteins inside cells.

Domains: Structural Biology [q-bio.BM]

Sarah Hostachy, Marie Masuda, Takayuki Miki, Itaru Hamachi, Sandrine Sagan, et al.. Graftable SCoMPIs enable the labeling and X-ray fluorescence imaging of proteins. Chemical Science, 2018, 9 (19), pp.4483-4487. ⟨10.1039/C8SC00886H⟩. ⟨hal-01905965⟩