Feeding Yellow Worms to Meagre: Effects on Whole-Body Fatty Acid Profile and Hepatic and Intestine Oxidative Status

Inês Guerreiro (1) , Carolina Castro (1) , Cláudia R Serra (1, 2) , Filipe Coutinho (1) , Ana Couto (1, 2) , Helena Peres (1, 2) , Pedro Pousão-Ferreira (3) , Geneviève Corraze (4) , Aires Oliva-Teles (1, 2) , Paula Enes (1, 2)

1 CIIMAR - Interdisciplinary Centre of Marine and Environmental Research [Matosinhos, Portugal]
2 Dpt of Biology [Porto]
3 IMPA - Portuguese Institute for Sea and Atmosphere
4 NuMéA - Nutrition, Métabolisme, Aquaculture

This study aimed to determine the effects of dietary inclusion of Tenebrio molitor larvae (yellow worms) meal (TM) on meagre fish (Argyrosomus regius) whole-body fatty acids (FA) profile and hepatic and intestine oxidative status. For that purpose, fish were fed for 9 weeks a fishmeal-based diet (control) or diets including 10%, 20%, or 30% TM. With the increase in dietary TM level, whole-body oleic acid, linoleic acid, monounsaturated FA, and n−6 polyunsaturated FA (PUFA) increased while saturated FA (SFA), n−3 PUFA, n−3 long chain-PUFA, SFA:PUFA ratio, n3:n6 ratio, and FA retention decreased. Hepatic superoxide dismutase (SOD), glucose-6-phosphate dehydrogenase (G6PDH), and glutathione reductase (GR) activities increased and catalase (CAT) and glutathione peroxidase (GPX) activities decreased with dietary TM inclusion. Hepatic total and reduced glutathione were lower in fish fed 20% TM. Intestinal CAT activity and oxidized glutathione increased and GPX activity decreased with dietary TM inclusion. Intestine SOD, G6PDH, and GR activities increased and malondialdehyde concentration decreased in fish fed the diets with lower TM inclusion levels. Liver and intestine oxidative stress index and liver malondialdehyde concentration were unaffected by dietary TM. In conclusion, to avoid major whole-body FA changes or antioxidant status imbalances, it is recommended to limit TM to 10% inclusion in meagre diets.

Mots clés: Argyrosomus regius; glutathione; insect meal; lipid peroxidation; oxidative stress enzymes; Tenebrio molitor

Domaines: Sciences du Vivant [q-bio]

Inês Guerreiro, Carolina Castro, Cláudia R Serra, Filipe Coutinho, Ana Couto, et al.. Feeding Yellow Worms to Meagre: Effects on Whole-Body Fatty Acid Profile and Hepatic and Intestine Oxidative Status. Antioxidants , 2023, 12 (5), pp.1031. ⟨10.3390/antiox12051031⟩. ⟨hal-04104973⟩