Molecular programming of the hepatic lipid metabolism via a parental high carbohydrate and low protein diet in rainbow trout

Therese Callet (1) , Hongyan Li (1, 2, 3) , Cécile Heraud (1) , Laurence Larroquet (1) , Anthony Lanuque (1) , Franck Sandres (1) , Frédéric Terrier (1) , Anne Surget (1) , Geneviève Corraze , Stéphane Panserat , Lucie Marandel

1 NuMéA - Nutrition, Métabolisme, Aquaculture
2 State Key Laboratory of Freshwater Ecology and Biotechnology
3 Pearl-River Fisheries Research Institute, Xo. 1 Xingyu Reoad, Liwan District, Guangzhou 510380, P. R. of China

It is now recognised that parental diets could alter their offspring metabolism, concept known as nutritional programming. For agronomic purposes, it has been previously proposed that programming could be employed as a strategy to prepare individual for future nutritional challenges. Concerning cultured fish that belong to high trophic level, plant-derived carbohydrates are a possible substitute for the traditional protein-rich fishmeal in broodstock diet, lowering thus the dietary protein-to-carbohydrate ratio (HC/LP nutrition). However, in mammals, numerous studies have previously demonstrated that parental HC/LP nutrition negatively affects their offspring in the long term. Therefore, the question of possible adaptation to plant-based diets, via parental nutrition, should be explored. First, the maternal HC/LP nutrition induced a global DNA hypomethylation in the liver of their offspring. Interestingly at the gene expression level, the effects brought by the maternal and paternal HC/LP nutrition cumulated in the liver, as indicated by the altered transcriptome. The paternal HC/LP nutrition significantly enhanced cholesterol synthesis at the transcriptomic level. Furthermore, hepatic genes involved in long-chain polyunsaturated fatty acids were significantly increased by the parental HC/LP nutrition, affecting thus both hepatic and muscle fatty acid profiles. Overall, the present study demonstrated that lipid metabolism could be modulated via a parental nutrition in rainbow trout, and that such modulations have consequences on their progeny phenotypes

Mots clés: Cholesterol metabolism; Global DNA methylation; Long-chain polyunsaturated fatty acids; Progeny

Domaines: Biologie animale; Alimentation et Nutrition

Therese Callet, Hongyan Li, Cécile Heraud, Laurence Larroquet, Anthony Lanuque, et al.. Molecular programming of the hepatic lipid metabolism via a parental high carbohydrate and low protein diet in rainbow trout. Animal, 2022, 16 (12), pp.100670. ⟨10.1016/j.animal.2022.100670⟩. ⟨hal-03959464⟩