Oxidative stress suppression in C . elegans by peptides from dogfish skin via regulation of transcription factors DAF-16 and HSF-1

Xiaoli Ma , Jiao Li , Yi Zhang (1) , Orcun Hacariz , Jianguo Xia , Benjamin Simpson , Zhuanhua Wang

1 IPREM - Institut des sciences analytiques et de physico-chimie pour l'environnement et les materiaux

Functional peptides were obtained via enzymatic hydrolysis of smooth dogfish (Mustelus canis) skin. The enzyme-assisted process was optimized to achieve high yield of smooth dogfish skin peptides (SDSP). Fractions of SDSP (MW [removed]10 kDa) showed in vitro antioxidant activities. The peptides <2 kDa (SDSP<2 kDa) significantly improved motility, reduced ROS and H2O2 levels of Caenorhabditis elegans, and increased its resistance to oxidative stress compared to the other peptide fractions. In vivo function of SDSP<2 kDa could be explained by their capacity to increase the expression of stress-response genes. The enhanced resistance to oxidative stress mediated by SDSP<2 kDa was dependent on DAF-16 and HSF-1. The amino acid residues and sequences of SDSP<2 kDa were characterized and revealed a higher content of hydrophobic versus polar amino acid contents. This study (especially the in vivo investigation) explored new potent antioxidant peptides derived from dogfish skin

Keywords: Amino acids; Antioxidants; Enzymatic hydrolysis; Oxidative stress; Transcription Engineering uncontrolled terms

Domains: Chemical Sciences; Material chemistry; Chemical Sciences; Polymers; Chemical Sciences; Theoretical and/or physical chemistry; Chemical Sciences; Analytical chemistry

Xiaoli Ma, Jiao Li, Yi Zhang, Orcun Hacariz, Jianguo Xia, et al.. Oxidative stress suppression in C . elegans by peptides from dogfish skin via regulation of transcription factors DAF-16 and HSF-1. Food and Function, 2022, 13 (2), pp.716-724. ⟨10.1039/d1fo02271g⟩. ⟨hal-03572490⟩