Metagenomics Versus Metatranscriptomics of the Murine Gut Microbiome for Assessing Microbial Metabolism During Inflammation - Université de Pau et des Pays de l'Adour Access content directly
Journal Articles Frontiers in Microbiology Year : 2022

Metagenomics Versus Metatranscriptomics of the Murine Gut Microbiome for Assessing Microbial Metabolism During Inflammation

Juan Jovel
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Aissata Nimaga
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Aducio Thiesen
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Naomi Hotte
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Michael Bording-Jorgensen
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Sudip Subedi
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Jessica Hamilton
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Eric J Carpenter
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Shokrollah Elahi
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Gane Ka-Shu Wong
Andrew L Mason
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Abstract

Shotgun Metagenomics Versus Metatranscriptomics profile bacterial population composition and taxa relative abundance, but did not inform about differential gene content associated with inflammation. Metatranscriptomics was more robust for capturing bacterial metabolism in real time. Although both approaches are complementary, it is often not possible to apply them in parallel. We hope our data will help researchers to decide which approach is more appropriate for the study of different aspects of the microbiome.
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Dates and versions

hal-03571948 , version 1 (14-02-2022)

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Juan Jovel, Aissata Nimaga, Tracy Jordan, Sandra O’keefe, Jordan Patterson, et al.. Metagenomics Versus Metatranscriptomics of the Murine Gut Microbiome for Assessing Microbial Metabolism During Inflammation. Frontiers in Microbiology, 2022, 13, ⟨10.3389/fmicb.2022.829378⟩. ⟨hal-03571948⟩
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