Metagenomics Versus Metatranscriptomics of the Murine Gut Microbiome for Assessing Microbial Metabolism During Inflammation

Juan Jovel; Aissata Nimaga; Tracy Jordan; Sandra O’keefe; Jordan Patterson; Aducio Thiesen; Naomi Hotte; Michael Bording-Jorgensen; Sudip Subedi; Jessica Hamilton; Eric Carpenter; Béatrice Lauga; Shokrollah Elahi; Karen Madsen; Gane Ka-Shu Wong; Andrew Mason

doi:10.3389/fmicb.2022.829378

Journal articles

Metagenomics Versus Metatranscriptomics of the Murine Gut Microbiome for Assessing Microbial Metabolism During Inflammation

Juan Jovel ^{1, *} Aissata Nimaga Tracy Jordan ¹ Sandra O’keefe ¹ Jordan Patterson ¹ Aducio Thiesen Naomi Hotte Michael Bording-Jorgensen Sudip Subedi Jessica Hamilton Eric Carpenter Béatrice Lauga ^{2, 3} Shokrollah Elahi Karen Madsen ¹ Gane Ka-Shu Wong Andrew Mason ^{1, *}

* Corresponding author

1 University of Alberta

2 UPPA - Université de Pau et des Pays de l'Adour

3 IPREM - Institut des sciences analytiques et de physico-chimie pour l'environnement et les materiaux

Abstract : Shotgun Metagenomics Versus Metatranscriptomics profile bacterial population composition and taxa relative abundance, but did not inform about differential gene content associated with inflammation. Metatranscriptomics was more robust for capturing bacterial metabolism in real time. Although both approaches are complementary, it is often not possible to apply them in parallel. We hope our data will help researchers to decide which approach is more appropriate for the study of different aspects of the microbiome.

Keywords : gut inflammation shotgun metagenomics a section of the journal Frontiers in Microbiology microbiome This article was submitted to Evolutionary and Genomic Microbiology metatranscriptomics bacterial metabolic pathways

Document type :

Journal articles

Domain :

Life Sciences [q-bio] / Animal biology / Veterinary medicine and animal Health

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https://hal-univ-pau.archives-ouvertes.fr/hal-03571948
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Submitted on : Monday, February 14, 2022 - 9:33:09 AM
Last modification on : Wednesday, May 4, 2022 - 3:34:02 PM
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Metagenomics Versus Metatranscriptomics of the Murine Gut Microbiome for Assessing Microbial Metabolism During Inflammation

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Metagenomics Versus Metatranscriptomics of the Murine Gut Microbiome for Assessing Microbial Metabolism During Inflammation

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