Metagenomics Versus Metatranscriptomics of the Murine Gut Microbiome for Assessing Microbial Metabolism During Inflammation

Juan Jovel (1) , Aissata Nimaga , Tracy Jordan (1) , Sandra O’keefe (1) , Jordan Patterson (1) , Aducio Thiesen , Naomi Hotte , Michael Bording-Jorgensen , Sudip Subedi , Jessica Hamilton , Eric J Carpenter , Béatrice Lauga (2, 3) , Shokrollah Elahi , Karen L Madsen (1) , Gane Ka-Shu Wong , Andrew L Mason (1)

1 University of Alberta
2 UPPA - Université de Pau et des Pays de l'Adour
3 IPREM - Institut des sciences analytiques et de physico-chimie pour l'environnement et les materiaux

Shotgun Metagenomics Versus Metatranscriptomics profile bacterial population composition and taxa relative abundance, but did not inform about differential gene content associated with inflammation. Metatranscriptomics was more robust for capturing bacterial metabolism in real time. Although both approaches are complementary, it is often not possible to apply them in parallel. We hope our data will help researchers to decide which approach is more appropriate for the study of different aspects of the microbiome.

Keywords: This article was submitted to Evolutionary and Genomic Microbiology; a section of the journal Frontiers in Microbiology microbiome; gut inflammation; shotgun metagenomics; metatranscriptomics; bacterial metabolic pathways

Domains: Life Sciences [q-bio]; Animal biology; Veterinary medicine and animal Health

Juan Jovel, Aissata Nimaga, Tracy Jordan, Sandra O’keefe, Jordan Patterson, et al.. Metagenomics Versus Metatranscriptomics of the Murine Gut Microbiome for Assessing Microbial Metabolism During Inflammation. Frontiers in Microbiology, 2022, 13, ⟨10.3389/fmicb.2022.829378⟩. ⟨hal-03571948⟩