Primers for 22 candidate genes for ecological adaptations in Brassicaceae

H. Kuittinen , M. Aguade , D. Charlesworth , A. Haan , Béatrice Lauga (1, 2, 3) , T. Mitchell-Olds , S. Oikarinen , S. Ramos-Onsins , B. Stranger , P. van Tienderen , O. Savolainen

1 Edin. - University of Edinburgh
2 UPPA - Université de Pau et des Pays de l'Adour
3 IPREM - Institut pluridisciplinaire de recherche sur l'environnement et les matériaux

There is an increasing interest in direct screening of polymorphisms at candidate loci to associate them with adaptations in natural situations. We report primers that amplify regions at 22 putatively orthologous functional loci in the family Brassicaceae: Arabidopsis thaliana, its two wild outcrossing relatives, and Brassica oleracea. Four groups of genes were known to have specific functions in the model species A. thaliana in ecologically important traits: timing of reproduction; metabolism of secondary compounds; defence against pathogens or light perception. In addition, genes of a fifth group were selected with no consideration of their function, as reference loci.

Mots clés: Arabidopsis; Candidate genes; Defence-genes; Flowering time; Molecular adaptation; Phenylpropanoid pathway; Phytochromes

Domaines: Biochimie, Biologie Moléculaire; Biodiversité

H. Kuittinen, M. Aguade, D. Charlesworth, A. Haan, Béatrice Lauga, et al.. Primers for 22 candidate genes for ecological adaptations in Brassicaceae. Molecular Ecology Notes, 2002, 2 (3), pp.258-262. ⟨10.1046/j.1471-8286.2002.00210.x⟩. ⟨hal-03540181⟩