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Isolation of a type 2 metallothionein-like gene preferentially expressed in the tapetum in Zea mays

Abstract : A Zea mays cDNA, MZm3-4, was isolated by differential screening of a cDNA library obtained from meiotic stage anthers against a cDNA of 3-week-old seedlings. Northern blot analysis of RNA from different maize tissues and from male reproductive organs at various developmental stages demonstrated expression of a single transcript in anthers, from the pollen mother cell stage through the uninucleated microspore stage. In situ hybridization to anther sections resulted in a distinct signal only in the tapetum. The MZm3-4 cDNA is 743 nucleotides in length and has an open reading frame encoding a protein of 75 amino acids. Sequence comparisons with various databases revealed that MZm3-4 exhibits high similarities with type 2 plant metallothioneins at both the nucleotide and the amino-acid level. Primer extension analysis indicated that MZm3-4 cDNA is deleted of 13 bp at the 5∞ end. Southern blot analysis showed that the MZm3-4 gene may be present in one or two copies in a Z. mays inbred line genome. This is the first report of the isolation of a type 2 metallothionein-like protein in maize. Moreover, the expression of this type 2 metallothionein-like gene is high in the male reproductive organs engaged in microsporogenesis.
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https://hal-univ-pau.archives-ouvertes.fr/hal-03538770
Contributor : Béatrice Lauga Connect in order to contact the contributor
Submitted on : Tuesday, January 25, 2022 - 4:47:29 PM
Last modification on : Wednesday, May 4, 2022 - 3:34:01 PM

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Laurence Charbonnel-Campaa, Béatrice Lauga, Daniel Combes. Isolation of a type 2 metallothionein-like gene preferentially expressed in the tapetum in Zea mays. Gene, Elsevier, 2000, 254 (1-2), pp.199-208. ⟨10.1016/S0378-1119(00)00266-3⟩. ⟨hal-03538770⟩

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