Development of molecular driven screening for desulfurizing microorganisms targeting the dszB desulfinase gene - Archive ouverte HAL Access content directly
Journal Articles Research in Microbiology Year : 2021

Development of molecular driven screening for desulfurizing microorganisms targeting the dszB desulfinase gene

(1, 2) , (2) , (1) , (2)
1
2

Abstract

COnsensus DEgenerate Hybrid Oligonucleotide Primers (CODEHOP) were developed for the detection of the dszB desulfinase gene (2′-hydroxybiphenyl-2-sulfinate desulfinase; EC 3.13.1.3) by polymerase chain reaction (PCR), which allow to reveal larger diversity than traditional primers. The new developed primers were used as molecular monitoring tool to drive a procedure for the isolation of desulfurizing microorganisms. The primers revealed a large dszB gene diversity in environmental samples, particularly in diesel-contaminated soil that served as inoculum for enrichment cultures. The isolation procedure using the dibenzothiophene sulfone (DBTO2) as sole sulfur source reduced drastically the dszB gene diversity. A dszB gene closely related to that carried by Gordonia species was selected. The desulfurization activity was confirmed by the production of desulfurized 2-hydroxybiphenyl (2-HBP). Metagenomic 16S rRNA gene sequencing showed that the Gordonia genus was represented at low abundance in the initial bacterial community. Such observation highlighted that the culture medium and conditions represent the bottleneck for isolating novel desulfurizing microorganisms. The new developed primers constitute useful tool for the development of appropriate cultural-dependent procedures, including medium and culture conditions, to access novel desulfurizing microorganisms useful for the petroleum industry.
Not file

Dates and versions

hal-03411447 , version 1 (02-11-2021)

Identifiers

Cite

Emmanuel Duval, Cristiana Cravo-Laureau, Line Poinel, Robert Duran. Development of molecular driven screening for desulfurizing microorganisms targeting the dszB desulfinase gene. Research in Microbiology, 2021, 172 (6), pp.103872. ⟨10.1016/j.resmic.2021.103872⟩. ⟨hal-03411447⟩
17 View
0 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More