Interactions of C. frondosa-derived inhibitory peptides against angiotensin I-converting enzyme (ACE), α-amylase and lipase

Yi Zhang (1, 2) , Shudong He , Xin Rui , Benjamin Simpson (2)

1 IPREM - Institut des sciences analytiques et de physico-chimie pour l'environnement et les materiaux
2 Department of Food Science and Agricultural Chemistry [Montréal]

The study illustrates the molecular mechanisms by which marine-derived peptides exhibited different structures and inhibition functions to concurrently inhibit multiple enzymes involved in chronic diseases. Peptides (2 mg/mL) exhibited inhibition against angiotensin-converting enzyme (ACE, inhibition of 52.2–78.8%), pancreatic α-amylase (16.3–27.2%) and lipase (5.3–17.0%). Further in silico analyses on physiochemistry, bioactivity, safety and interaction energy with target enzymes indicated that one peptide could inhibit multiple enzymes. Peptide FENLLEELK potent in inhibiting both ACE and α-amylase showed different mechanisms: it had ordered extended structure in ACE active pocket with conventional H-bond towards Arg522 which is the ligand for activator Cl-, while the peptide folded into compact “lariat” conformation within α-amylase active site and the K residue in peptide formed intensive H-bonds and electrostatic interactions with catalytic triad Asp197 − Asp300 − Glu233. Another peptide APFPLR showed different poses in inhibiting ACE, α-amylase and lipase, and it formed direct interactions to lipase catalytic residues Phe77 & His263.

Mots clés: ACE; Inhibition; Lipase; Peptides; α-Amylase

Domaines: Chimie

Yi Zhang, Shudong He, Xin Rui, Benjamin Simpson. Interactions of C. frondosa-derived inhibitory peptides against angiotensin I-converting enzyme (ACE), α-amylase and lipase. Food Chemistry, 2022, 367, pp.130695. ⟨10.1016/j.foodchem.2021.130695⟩. ⟨hal-03328917⟩