Does selenium fortification of kale and kohlrabi sprouts change significantly their biochemical and cytotoxic properties?

Paweł Zagrodzki (1) , Paweł Paśko (1) , Agnieszka Galanty (1) , Małgorzata Tyszka-Czochara (1) , Renata Wietecha-Posłuszny (1) , Pol Salvans Rubió (2) , Henryk Bartoń (1) , Ewelina Prochownik (1) , Bożena Muszyńska (1) , Katarzyna Sułkowska-Ziaja (1) , Katarzyna Bierla (3) , Ryszard Lobinski (3, 4) , Joanna Szpunar (3) , Shela Gorinstein (5)

1 UJ - Uniwersytet Jagielloński w Krakowie = Jagiellonian University
2 UB - Universitat de Barcelona
3 IPREM - Institut des sciences analytiques et de physico-chimie pour l'environnement et les materiaux
4 Sechenov First Moscow State Medical University
5 HUJ - The Hebrew University of Jerusalem

Background: The sprouts of Brassica vegetables are known from their nutritional and chemopreventive values. Moreover, sprouts fortification with some trace elements, like selenium, may increase their importance in human diet. Thus, the aim of our study was to examine if selenium enrichment of kale and kohlrabi sprouts may influence their biochemical properties (phenolic acids and L-tryptophan content, antioxidant potential) or cytotoxic activity. Additional aim of the study was to evaluate the profile of selenium compounds and to describe the multidimensional interactions between the mentioned parameters. Methods: Selenium content in the sprouts was evaluated by double-channel atomic fluorescence spectrometer AFS-230 with the flow hydride-generation system. Separation of selenium species in water soluble fraction was performed by size-exclusion LC-ICP-MS. The identification and quantification of phenolic acids and L-tryptophan was performed by HPLC. For antioxidant activity DPPH and FRAP methods were used. Cytotoxic activity of the sprouts extracts on a panel of human metastatic carcinoma cells was evaluated by MTT test. Results: Selenium content in the fortified sprouts was several orders of magnitude higher than in the unfortified ones. Only small percentage of supplemented selenium (ca. 10 %) was incorporated into the sprouts as seleno-Lmethionine, while the other detected selenium species remained unidentified. Selenium fortification differently stimulated the production of phenolic acids (sinapic, chlorogenic, isochlorogenic and caffeic acid) in the tested sprouts, depending on the particular species, selenium dose and the investigated compound. PCA analysis revealed strong correlation between antioxidant parameters and phenolic acids and L-tryptophan, while Se correlated only with caffeic acid. The sprouts extracts (≥1 mg/mL) showed cytotoxic potency to all the studied cancer cell lines (SW480, SW620, HepG2, SiHa), regardless the selenium supplementation. Conclusion: Se-fortified kale and kohlrabi sprouts are good candidates for functional food ingredients. Moreover, these results indicate that the sprouts enriched with sodium selenite show higher nutritional value, without significant changes in their cytotoxic activity.

Mots clés: Selenium; Sprouts; Phenolic acids; Speciation analysis; Cytotoxic activity

Domaines: Alimentation et Nutrition; Ingénierie des aliments; Chimie analytique

Paweł Zagrodzki, Paweł Paśko, Agnieszka Galanty, Małgorzata Tyszka-Czochara, Renata Wietecha-Posłuszny, et al.. Does selenium fortification of kale and kohlrabi sprouts change significantly their biochemical and cytotoxic properties?. Journal of Trace Elements in Medicine and Biology, 2020, 59, pp.126466. ⟨10.1016/j.jtemb.2020.126466⟩. ⟨hal-03133477⟩