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Journal Articles Metallomics Year : 2020

Water soluble selenometabolome of Cardamine violifolia

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Eszter Borbála Both
  • Function : Author
Jiqian Xiang
  • Function : Author
Hongqing Yin
  • Function : Author
Yu Kang
  • Function : Author
Shuxun Shao
  • Function : Author
Katalin Kiszelák
  • Function : Author
Zsuzsa Jókai
  • Function : Author

Abstract

Low molecular weight selenium containing metabolites in the leaves of the selenium hyperaccumulator Cardamine violifolia (261 mg total Se per kg d.w.) were targeted in this study. One dimensional cation exchange chromatography coupled to ICP-MS was used for purification and fractionation purposes prior to LC-Unispray-QTOF-MS analysis. The search for selenium species in full scan spectra was assisted with an automated mass defect based filtering approach. Besides selenocystathionine, selenohomocystine and its polyselenide derivative, a total number of 35 water soluble selenium metabolites other than selenolanthionine were encountered, including 30 previously unreported compounds. High occurrence of selenium containing hexoses was observed, together with the first assignment of N-glycoside derivatives of selenolanthionine. Quantification of the most abundant selenium species, selenolanthionine, was carried out with an ion pairing LC-post column isotope dilution ICP-MS setup, which revealed that this selenoamino acid accounted for 30% of the total selenium content of the leaf (78 mg (as Se) per kg d.w.).

Dates and versions

hal-03113700 , version 1 (18-01-2021)

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Laurent Ouerdane, Eszter Borbála Both, Jiqian Xiang, Hongqing Yin, Yu Kang, et al.. Water soluble selenometabolome of Cardamine violifolia. Metallomics, 2020, 12 (12), pp.2032-2048. ⟨10.1039/d0mt00216j⟩. ⟨hal-03113700⟩
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