Potential of Fourier Transform Mass Spectrometry (Orbitrap and Ion Cyclotron Resonance) for Speciation of the Selenium Metabolome in Selenium-Rich Yeast - Archive ouverte HAL Access content directly
Journal Articles Frontiers in Chemistry Year : 2020

Potential of Fourier Transform Mass Spectrometry (Orbitrap and Ion Cyclotron Resonance) for Speciation of the Selenium Metabolome in Selenium-Rich Yeast

(1) , , , (1) , (1)
1

Abstract

The evolution of the field of element speciation, from the targeted analysis for specific element species toward a global exploratory analysis for the entirety of metal- or metalloid-related compounds present in a biological system (metallomics), requires instrumental techniques with increasing selectivity and sensitivity. The selectivity of hyphenated techniques, combining chromatography, and capillary electrophoresis with element-specific detection (usually inductively coupled plasma mass spectrometry, ICP MS), is often insufficient to discriminate all the species of a given element in a sample. The necessary degree of specificity can be attained by ultrahigh-resolution (R >100,000 in the m/z < 1,000 range for a 1 s scan) mass spectrometry based on the Fourier transformation of an image current of the ions moving in an Orbitrap or an ion cyclotron resonance (ICR) cell. The latest developments, allowing the separate detection of two ions differing by a mass of one electron (0.5 mDa) and the measurement of their masses with a sub-ppm accuracy, make it possible to produce comprehensive lists of the element species present in a biological sample. Moreover, the increasing capacities of multistage fragmentation often allow their de novo identification. This perspective paper critically discusses the potential state-of-the-art of implementation, and challenges in front of FT (Orbitrap and ICR) MS for a large-scale speciation analysis using, as example, the case of the metabolism of selenium by yeast.
Fichier principal
Vignette du fichier
fchem-08-612387.pdf (5.42 Mo) Télécharger le fichier
Origin : Publisher files allowed on an open archive

Dates and versions

hal-03095520 , version 1 (04-01-2021)

Identifiers

Cite

Katarzyna Bierla, Giovanni Chiappetta, Joelle Vinh, Ryszard Lobinski, Joanna Szpunar. Potential of Fourier Transform Mass Spectrometry (Orbitrap and Ion Cyclotron Resonance) for Speciation of the Selenium Metabolome in Selenium-Rich Yeast. Frontiers in Chemistry, 2020, 8, ⟨10.3389/fchem.2020.612387⟩. ⟨hal-03095520⟩
30 View
64 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More