Microbial Communities and Sulfate-Reducing Microorganisms Abundance and Diversity in Municipal Anaerobic Sewage Sludge Digesters from a Wastewater Treatment Plant (Marrakech, Morocco)

Abdelaziz El Houari (1, 2) , Magali Ranchou-Peyruse (1) , Anthony Ranchou-Peyruse (1) , Rhizlane Bennisse (2) , Radia Bouterfas (2) , Maria Goni (1) , Abdel-Ilah Qatibi (2) , Remy Guyoneaud (1)

1 IPREM - Institut des sciences analytiques et de physico-chimie pour l'environnement et les materiaux
2 UCA - Université Cadi Ayyad [Marrakech]

Both molecular analyses and culture-dependent isolation were combined to investigate the diversity of sulfate-reducing prokaryotes and explore their role in sulfides production in full-scale anaerobic digesters (Marrakech, Morocco). At global scale, using 16S rRNA gene sequencing, Proteobacteria, Bacteroidetes, Firmicutes, Actinobacteria, Synergistetes, and Euryarchaeota were the most dominant phyla. The abundance of Archaea (3.1-5.7%) was linked with temperature. The mcrA gene ranged from 2.18 × 10 5 to 1.47 × 10 7 gene copies.g −1 of sludge. The sulfate-reducing prokaryotes, representing 5% of total sequences, involved in sulfides production were Peptococcaceae, Syntrophaceae, Desulfobulbaceae, Desulfovibrionaceae, Syntrophobacteraceae, Desulfurellaceae, and Desulfobacteraceae. Furthermore, dsrB gene ranged from 2.18 × 10 5 to 1.92 × 10 7 gene copies.g −1 of sludge. The results revealed that exploration of diversity and function of sulfate-reducing bacteria may play a key role in decreasing sulfide production, an undesirable by-product, during anaerobic digestion.

Mots clés: anaerobic digestion; microbial diversity; sulfate-reducing bacteria; Archaea; sulfides; biogas

Domaines: Biotechnologies; Ingénierie de l'environnement

Abdelaziz El Houari, Magali Ranchou-Peyruse, Anthony Ranchou-Peyruse, Rhizlane Bennisse, Radia Bouterfas, et al.. Microbial Communities and Sulfate-Reducing Microorganisms Abundance and Diversity in Municipal Anaerobic Sewage Sludge Digesters from a Wastewater Treatment Plant (Marrakech, Morocco). Processes, 2020, 8 (10), pp.1284. ⟨10.3390/pr8101284⟩. ⟨hal-02985369⟩