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Reverse-transcriptase quantitative PCR method to detect uptake of hydrogen produced from cyanobacteria by Alcaligenes hydrogenophilus, an aerobic hydrogen-oxidising bacterium

Abstract : Hydrogen-oxidising bacteria play a key ecological role in a variety of habitats including the rhizosphere and hot springs. To investigate the possibly of interspecies hydrogen exchange between cyanobacteria and hydrogen-oxidising bacteria, we developed a sensitive and reliable reverse-transcriptase qPCR assay for up-regulation of the hupS gene in the knallgas bacterium Alcaligenes hydrogenophilus DSM2625. The assay detected up-regulation of the gene at initial hydrogen concentrations as low as 0.12 μM. Expression of hupS also increased in the presence of hydrogen-producing cyanobacteria, both when Ah DSM2625 was directly added to a hydrogen-producing culture of the cyanobacteria, and when cultures were physically separated in a vessel that allowed gas exchange. Additional refinements and development of the sensitive assay will lead to a better understanding of hydrogen exchange in aerobic ecosystems and development of reporter strains to detect hydrogen-producing organisms.
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https://hal-univ-pau.archives-ouvertes.fr/hal-02282497
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Submitted on : Tuesday, September 10, 2019 - 9:28:37 AM
Last modification on : Wednesday, October 21, 2020 - 3:34:53 AM

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Sarah Schroeder, Anthony Ranchou-Peyruse, Magali Ranchou-Peyruse, Jim Spain. Reverse-transcriptase quantitative PCR method to detect uptake of hydrogen produced from cyanobacteria by Alcaligenes hydrogenophilus, an aerobic hydrogen-oxidising bacterium. Archives of Microbiology, Springer Verlag, 2011, 193 (9), pp.687-692. ⟨10.1007/s00203-011-0714-9⟩. ⟨hal-02282497⟩

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