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HALathon : 25 mai - 19 juin 2021 |  |
Journal articles
Reverse-transcriptase quantitative PCR method to detect uptake of hydrogen produced from cyanobacteria by Alcaligenes hydrogenophilus, an aerobic hydrogen-oxidising bacterium
Abstract : Hydrogen-oxidising bacteria play a key ecological role in a variety of habitats including the rhizosphere and hot springs. To investigate the possibly of interspecies hydrogen exchange between cyanobacteria and hydrogen-oxidising bacteria, we developed a sensitive and reliable reverse-transcriptase qPCR assay for up-regulation of the hupS gene in the knallgas bacterium Alcaligenes hydrogenophilus DSM2625. The assay detected up-regulation of the gene at initial hydrogen concentrations as low as 0.12 μM. Expression of hupS also increased in the presence of hydrogen-producing cyanobacteria, both when Ah DSM2625 was directly added to a hydrogen-producing culture of the cyanobacteria, and when cultures were physically separated in a vessel that allowed gas exchange. Additional refinements and development of the sensitive assay will lead to a better understanding of hydrogen exchange in aerobic ecosystems and development of reporter strains to detect hydrogen-producing organisms.
https://hal-univ-pau.archives-ouvertes.fr/hal-02282497
Contributor : Sylvie Blanc <>
Submitted on : Tuesday, September 10, 2019 - 9:28:37 AM
Last modification on : Tuesday, February 2, 2021 - 2:58:03 PM
Contributor : Sylvie Blanc <>
Submitted on : Tuesday, September 10, 2019 - 9:28:37 AM
Last modification on : Tuesday, February 2, 2021 - 2:58:03 PM
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