Cloning vectors and antibiotic-resistance markers for Brevibacterium sp. R312

C.K.N.C.K. Chion , Robert Duran (1) , A. Arnaud , P. Galzy

1 IPREM - Institut des sciences analytiques et de physico-chimie pour l'environnement et les materiaux

Replication of several cryptic plasmids from coryneform strains was investigated in Brevibacterium sp. R312. Only the Corynebacterium glutamicum pSR1 replicon was found to be suitable for establishing a host-vector system. Two pSR1 derivatives, pRPCG200 and pHYCG1, were used as cloning vectors. They carry a neomycin-resistance-encoding and a tetracycline-resistance-encoding gene, respectively.

Domaines: Chimie

C.K.N.C.K. Chion, Robert Duran, A. Arnaud, P. Galzy. Cloning vectors and antibiotic-resistance markers for Brevibacterium sp. R312. Gene, 1991, 105 (1), pp.119-124. ⟨10.1016/0378-1119(91)90522-D⟩. ⟨hal-01841812⟩