Isolation of promoter sequences from Brevibacterium sp. R312.

Robert Duran (1) , C.K. Chion , A. Arnaud , P. Galzy

1 IPREM - Institut des sciences analytiques et de physico-chimie pour l'environnement et les materiaux

Promoter sequences recognized by Escherichia coli RNA polymerase were isolated from Brevibacterium sp. R312, a coryneform strain producing nitrile hydratase and amidase. Ten Escherichia coli clones containing promoter sequences were selected for their ability to grow with chloramphenicol concentrations of up to 1500 micrograms/ml. The strength of these promoter sequences was determined. We carried out a preliminary study of the strongest promoter having a chloramphenicol acetyl-transferase/beta-lactamase activities ratio of 18.4.

Domaines: Chimie

Robert Duran, C.K. Chion, A. Arnaud, P. Galzy. Isolation of promoter sequences from Brevibacterium sp. R312.. Zentralblatt für Mikrobiologie, 1992, 147 (8), pp.499-502. ⟨hal-01841809⟩