The N-terminal amino acid sequences of Brevibacterium sp. R312 nitrile hydratase

Robert Duran (1) , C.K.N.C.K. Chion , F. Bigey , A. Arnaud , P. Galzy

1 IPREM - Institut des sciences analytiques et de physico-chimie pour l'environnement et les materiaux

Nitrile hydratase from Brevibacterium sp. R312 was purified to homogeneity. The isoelectric point was 5.75. The two kinds of subunits were separated by reverse phase HPLC and their N-terminal amino acid sequences were found to be identical to those of Rhodococcus sp. N-774 nitrile hydratase

Domaines: Chimie

Robert Duran, C.K.N.C.K. Chion, F. Bigey, A. Arnaud, P. Galzy. The N-terminal amino acid sequences of Brevibacterium sp. R312 nitrile hydratase. Applied Microbiology and Biotechnology, 1992, 32 (1), pp.13-19. ⟨10.1002/jobm.3620320104⟩. ⟨hal-01841807⟩