Rhodococcus pyridinovorans MW3, a bacterium producing a nitrile hydratase

S. Precigou , M. Wieser , P. Pommares , P. Goulas , Robert Duran (1)

1 IPREM - Institut des sciences analytiques et de physico-chimie pour l'environnement et les materiaux

Rhodococcus pyridinovorans MW3 was isolated from an arable land of manioc from the Congo for its ability to transform acrylonitrile to acrylamide. This strain contains a cobalt nitrile hydratase (NHase) showing high sequence homology with NHases so far described. The specific NHase activity was 97 U mg-1 dry wt. NHase production by R. pyridinovorans MW3 was urea and Co-dependent. The NHase was active for acrylamide up to 60% (w/v) indicating its potential for acrylamide production

Domaines: Chimie

S. Precigou, M. Wieser, P. Pommares, P. Goulas, Robert Duran. Rhodococcus pyridinovorans MW3, a bacterium producing a nitrile hydratase. Biotechnology Letters, 2004, 26 (17), pp.1379-1384. ⟨10.1023/B:BILE.0000045636.47527.99⟩. ⟨hal-01841793⟩