Full-field transmission X-ray microscopy for bio-imaging

J.C. Andrews , S. Brennan , Y. Liu , P. Pianetta (1) , E.A.C. Almeida , M.C.H. van Der Meulen , Z. Wu , Z. Mester , Laurent Ouerdane (2) , J. Gelb , M. Feser , J. Rudati , A. Tkachuk , W. Yun

1 Stanford University
2 IPREM - Institut des sciences analytiques et de physico-chimie pour l'environnement et les materiaux

A full-field hard-x-ray microscope at SSRL has successfully imaged samples of biological and environmental origin at 40 nm resolution. Phase contrast imaging of trabeculae from a female mouse tibia, loaded in vivo to study the effects of weight-bearing on bone structure, revealed a complex network of osteocytes and canaliculi. Imaging of cordgrass roots exposed to mercury revealed nanoparticles with strong absorption contrast. 3D tomography of yeast cells grown in selenium rich media showed internal structure.

Domaines: Chimie analytique

J.C. Andrews, S. Brennan, Y. Liu, P. Pianetta, E.A.C. Almeida, et al.. Full-field transmission X-ray microscopy for bio-imaging. Journal of Physics: Conference Series, 2009, 186, ⟨10.1088/1742-6596/186/1/012081⟩. ⟨hal-01649688⟩