Simultaneous speciation of selenomethionine and 2-hydroxy-4-methylselenobutanoic acid by HPLC-ICP MS in biological samples

V. Vacchina (1) , M. Moutet , J.-C. Yadan , F. De Baene , B. Kudla , Ryszard Lobinski (1)

1 IPREM - Institut des sciences analytiques et de physico-chimie pour l'environnement et les materiaux

An analytical method was developed for the simultaneous speciation of selenomethionine (SeMet) and 2-hydroxy-4-methylselenobutanoic acid (NutraSelen®), a new SeMet precursor. The compounds could be baseline resolved by ion-pairing reversed-phase HPLC using ICP MS detection. Detection limits of 1 ng mL-1 (Se content) could be reached. SELM-1 reference material was used to validate the SeMet measurement. Additionally, the quantification of NutraSelen® was validated by standard addition together with checking the Se mass balance. The procedure developed was then applied to the monitoring of the conversion of NutraSelen® into SeMet by yeast. © 2010 Elsevier B.V. All rights reserved.

Mots clés: HPLC-ICP MS; NutraSelen®; Selenium; Selenomethionine

Domaines: Chimie; Chimie analytique

V. Vacchina, M. Moutet, J.-C. Yadan, F. De Baene, B. Kudla, et al.. Simultaneous speciation of selenomethionine and 2-hydroxy-4-methylselenobutanoic acid by HPLC-ICP MS in biological samples. Journal of Chromatography B - Analytical Technologies in the Biomedical and Life Sciences, 2010, 878 (15-16), pp.1178--1180. ⟨10.1016/j.jchromb.2010.03.002⟩. ⟨hal-01564528⟩