Multimode detection (LA-ICP-MS, MALDI-MS and nanoHPLC-ESI-MS2) in 1D and 2D gel electrophoresis for selenium-containing proteins

G. Ballihaut , Christophe Pécheyran (1) , Sandra Mounicou (1) , Hugues Preud'Homme (2) , R. Grimaud , Ryszard Lobinski (1)

1 LCABIE - Laboratoire de Chimie Analytique Bio-Inorganique et Environnement
2 IPREM - Institut des sciences analytiques et de physico-chimie pour l'environnement et les materiaux

Speciation of heteroelement-containing proteins essential for human health is one of the most rapidly developing areas in bio-inorganic analytical chemistry (metallomics). We discuss recent advances in mass spectrometry (MS) techniques for detection and identification of selenium (Se)-containing proteins separated by gel electrophoresis (GE). These advances include location of Se-containing bands in 1D GE or spots in 2D GE by laser-ablation inductively coupled plasma MS (LA-ICP-MS) followed by protein identification by matrix-assisted laser-desorption/ionization MS (MALDI-MS) and nano-high-performance liquid chromatography-electrospray-tandem MS (nanoHPLC-ESI-MS2). We highlight the differences with the classical proteomics approach resulting from the presence of the Se atom. © 2007 Elsevier Ltd. All rights reserved.

Domaines: Chimie analytique

G. Ballihaut, Christophe Pécheyran, Sandra Mounicou, Hugues Preud'Homme, R. Grimaud, et al.. Multimode detection (LA-ICP-MS, MALDI-MS and nanoHPLC-ESI-MS2) in 1D and 2D gel electrophoresis for selenium-containing proteins. Trends in Analytical Chemistry, 2007, 26 (3), pp.183-190. ⟨10.1016/j.trac.2007.01.007⟩. ⟨hal-01560354⟩