Study of the polymorphism of metallothioneins by ion-spray mass spectrometry

Etude du polymorphisme des metallothioneines par spectrometrie de masse à source ion-spray

H. Chassaigne (1) , Ryszard Lobinski (1)

1 LCABIE - Laboratoire de Chimie Analytique Bio-Inorganique et Environnement

Ion-spray mass spectrometry (IS-MS) is shown to be an attractive technique to identify and to differentiate among the sub-isoforms of metallothioneins (MT). Different modes of application of IS-MS are discussed using the example of MT-2 rabbit liver prepurified by size-exclusion and anion-exchange chromatography. Direct IS-MS mode, microbore HPLC with on- line IS-MS detection of fully cadmiated (Cd7-MT isoforms), HPLC with post- column acidification and detection of the apo-MT isoforms by IS-MS, and with post-column acidification and detection of the Cd ion by IS-MS are discussed.

Domaines: Chimie analytique

H. Chassaigne, Ryszard Lobinski. Etude du polymorphisme des metallothioneines par spectrometrie de masse à source ion-spray. Analusis, 1998, 26 (6), pp.65-67. ⟨hal-00291459⟩