Investigation of the response of wood-rotting fungi to copper stress by size-exclusion chromatography and capillary zone electrophoresis with ICP MS detection

V. Vacchina (1) , P. Baldrian , J. Gabriel , J. Szpunar (1)

1 LCABIE - Laboratoire de Chimie Analytique Bio-Inorganique et Environnement

A method based on the coupling of size-exclusion chromatography (SEC) with inductively coupled plasma mass spectrometry (ICP MS) was developed for screening the changes in the bioligand composition of wood-rotting fungi as a function of their exposure to copper stress. Strains of four different species of wood-rotting fungi: Phanerochaete chrysosporium, Schizophyllum commune, Daedalea quercina and Pleurotus ostreatus were examined. Only one, namely Ph. chrysosporium, showed any significant difference in terms of the fingerprint of Cu-binding ligands between control and exposed cells which suggest trapping of Cu(II) by cell walls as the only resistance mechanisms. In the case of Ph. chrysosporium the bioinduction of a new Cu-binding ligand was demonstrated. The presence of a new compound in the SE chromatographic fraction of interest was confirmed by CZE-ICP MS. Attempts to identify the new compound by electrospray MS/MS failed because of insufficient sensitivity. © Springer-Verlag 2001.

Mots clés: Coupled techniques; CZE; ICP MS; Heavy-metal stress wood; HPLC; Rotting fungi

Domaines: Chimie analytique

V. Vacchina, P. Baldrian, J. Gabriel, J. Szpunar. Investigation of the response of wood-rotting fungi to copper stress by size-exclusion chromatography and capillary zone electrophoresis with ICP MS detection. Analytical and Bioanalytical Chemistry, 2002, 372 (3), pp.453-456. ⟨10.1007/s00216-001-1104-y⟩. ⟨hal-00291378⟩